In a fantastic collaboration with the lab of Thomas Pucadyil from IISER Pune, we investigated the role of Dynamin-related protein 1 (Drp1) and Dnm2 in tubule constriction and membrane fission in both mamalian cell systems and reconstitution experiments.
We found that Dnm2 is dispensable for peroxisomal fission, while Drp1 is essential for severing both mitochondrial and peroxisomal membranes. In addition, Drp1 can both constrict and sever reconstituted membrane tubles up to a radius of 250 nm, while Dnm2 displays a restricted fission capability even on thin (50 nm radius) tubes.
Many thanks to all members of both labs for their great work and effort for this publication!
Link to article can be found here.