Ryan Lab Methods Protocol

Please note: Ensure to wear appropriate personal protection equipment at all times during conduction experiments. Check the local workspace guidelines for laboratory work in place in your institute / department as well as the MSDS of chemical used in the experiments.

Buffer List

Mitochondrial Isolation from Cultured Cells

Mitochondrial Isolation from Cultured Cells (The Spanish Way)

Blue Native-PAGE

Colloidal Coomassie Staining

SDS-PAGE using Glycine Buffer

SDS-PAGE using Tris-Tricine system (& Gradient)

Western Transfer

Generation of knockout cell lines using CRISPR/Cas9